Composite

Part:BBa_K3396016:Design

Designed by: Changtai Xiao   Group: iGEM20_NUDT_CHINA   (2020-10-26)


CMV-replaceable-Fluc-P2A-Rluc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 871
    Illegal NgoMIV site found at 2215
    Illegal NgoMIV site found at 2236
    Illegal AgeI site found at 1939
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3102
    Illegal SapI.rc site found at 2121


Design Notes

The Replaceable region flanked with BsmBI was added into the plasmid to be easily replaced by other coding sequences.


Source

Fluc was originally expressed in Firefly, Rluc was originally expressed in Renilla reniformis.

References